A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays
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چکیده
منابع مشابه
Inhibitory effects associated with use of modified Photinus pyralis and Renilla reniformis luciferase vectors in dual reporter assays and implications for analysis of ISGs.
Luciferase reporter constructs are widely used for analysis of gene regulation when characterizing promoter and enhancer elements. We report that the recently developed codon-modified Renilla luciferase construct included as an internal standard for cotransfection must be used with great caution with respect to the amount of DNA transfected. Also, the dual-luciferase reporter vectors encoding P...
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Enzymes are deformable molecules: the molecular structure can exist in essentially a continuum of different conformations. These are accessible by applying mechanical stress to the molecule. Mechanics then provides a general molecular mechanism to modulate enzymatic reactions, by applying mechanical stresses which deform the enzyme. There are approximately 10 known different enzymatic reactions...
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The Renilla bioluminescent system in vivo is comprised of three proteins--the luciferase, green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called luciferin-binding protein (LBP). This work reports the cloning of the full-size cDNA encoding CBP from soft coral Renilla muelleri, its overexpression and properties of the recombinant protein. The apo-CBP was quantitati...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2006
ISSN: 0003-2697
DOI: 10.1016/j.ab.2006.04.046